Abstract
Objective
To investigate the therapeutic effect of low intensity pulsed ultrasound (LIPUS) in
a stress urinary incontinence (SUI) rat model and its influence on myogenic satellite
cells.
Methods
Fifty Sprague-Dawley rats underwent vaginal distension and bilateral ovariectomy mimicking
partum injury and menopause to construct SUI models, which were further randomized
into 100 mW/cm2 LIPUS, 200 mW/cm2 LIPUS, 300 mW/cm2 LIPUS, and none-treatment control subgroups with 10 rats per subgroup. Ten rats served
as mock operation control. Leak point pressure and bladder capacity were recorded
1 week after LIPUS treatment. Immunofluorescence staining and Western blot were performed
to examine histological changes, myodifferentiation, and signaling pathway.
Results
Here,we found the leak point pressure and bladder capacity were restored in 200 mW/cm2 LIPUS and 300 mW/cm2 LIPUS groups, but not in 100 mW/cm2 LIPUS group. More robust striated muscle regeneration was observed in 200 mW/cm2 LIPUS group comparing with the SUI none-treatment group. Moreover, we found LIPUS
activated the myodifferentiation of muscle satellite cells, which is correlated to
p38 phosphorylation level.
Conclusion
LIPUS restored the leak point pressure and bladder capacity, and activated satellite
cell myodifferentiation in SUI rat model.
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Article Info
Publication History
Published online: September 28, 2018
Accepted:
September 18,
2018
Received in revised form:
September 8,
2018
Received:
June 22,
2018
Footnotes
Conflict of Interest Statement: None declared.
Identification
Copyright
© 2018 Published by Elsevier Inc.
