A HindIII Polymorphism of Fibronectin Gene Is Associated With Nephrolithiasis

Onaran, Metin; Yılmaz, Akın; Şen, İlker; Ergun, Mehmet Ali; Çamtosun, Ahmet; Küpeli, Bora; Menevşe, Sevda; Bozkırlı, İbrahim

doi:10.1016/j.urology.2009.05.010

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Volume 74, Issue 5 , Pages 1004-1007, November 2009

A HindIII Polymorphism of Fibronectin Gene Is Associated With Nephrolithiasis

Metin Onaran
- Department of Urology, School of Medicine, Gazi University, Ankara, Turkey
- Reprint requests: Metin Onaran, M.D., Department of Urology, School of Medicine, Gazi University, 06500 Besevler, Ankara, Turkey
Akın Yılmaz
- Department of Medical Biology and Genetics, School of Medicine, Gazi University, Ankara, Turkey
İlker Şen
- Department of Urology, School of Medicine, Gazi University, Ankara, Turkey
Mehmet Ali Ergun
- Department of Medical Genetics, School of Medicine, Gazi University, Ankara, Turkey
Ahmet Çamtosun
- Department of Urology, School of Medicine, Gazi University, Ankara, Turkey
Bora Küpeli
- Department of Urology, School of Medicine, Gazi University, Ankara, Turkey
Sevda Menevşe
- Department of Medical Biology and Genetics, School of Medicine, Gazi University, Ankara, Turkey
İbrahim Bozkırlı
- Department of Urology, School of Medicine, Gazi University, Ankara, Turkey

Received 6 March 2009; accepted 5 May 2009. published online 17 July 2009.

Objectives

To evaluate the association between fibronectin gene (FN1) polymorphisms and calcium oxalate nephrolithiasis as a genetic risk factor.

Methods

Genomic DNA of 143 patients with calcium oxalate nephrolithiasis and 154 healthy controls were screened for polymorphisms (HaeIII b, MspI, and HindIII) of the FN1 gene, using polymerase chain reaction-restriction fragments length polymorphism method. Allele and genotype frequencies were compared between the groups.

Results

Although the observed differences between distribution of genotypes of AA, AB, and BB (for HaeIII b), as well as CC, CD, and DD (MspI) were not significant, FF genotype for HindIII showed significant difference when compared with both EF and EE + EF genotype (P = .00202 and P = .00203, respectively).

Conclusions

The results of our study revealed that HindIII polymorphism of the FN1 gene is highly associated with calcium oxalate stone disease. This association makes FN a good candidate for further studies about the etiology of stone disease, and in the future it could be a candidate marker for evaluating the genetic risks in patients with nephrolithiasis.