A HindIII Polymorphism of Fibronectin Gene Is Associated With Nephrolithiasis
Received 6 March 2009; accepted 5 May 2009. published online 17 July 2009.
Objectives
To evaluate the association between fibronectin gene (FN1) polymorphisms and calcium oxalate nephrolithiasis as a genetic risk factor.
Methods
Genomic DNA of 143 patients with calcium oxalate nephrolithiasis and 154 healthy controls were screened for polymorphisms (HaeIII b, MspI, and HindIII) of the FN1 gene, using polymerase chain reaction-restriction fragments length polymorphism method. Allele and genotype frequencies were compared between the groups.
Results
Although the observed differences between distribution of genotypes of AA, AB, and BB (for HaeIII b), as well as CC, CD, and DD (MspI) were not significant, FF genotype for HindIII showed significant difference when compared with both EF and EE + EF genotype (P = .00202 and P = .00203, respectively).
Conclusions
The results of our study revealed that HindIII polymorphism of the FN1 gene is highly associated with calcium oxalate stone disease. This association makes FN a good candidate for further studies about the etiology of stone disease, and in the future it could be a candidate marker for evaluating the genetic risks in patients with nephrolithiasis.
aDepartment of Urology, School of Medicine, Gazi University, Ankara, Turkey
bDepartment of Medical Biology and Genetics, School of Medicine, Gazi University, Ankara, Turkey
cDepartment of Medical Genetics, School of Medicine, Gazi University, Ankara, Turkey
Reprint requests: Metin Onaran, M.D., Department of Urology, School of Medicine, Gazi University, 06500 Besevler, Ankara, Turkey
ABSTRACT