Urology
Volume 64, Issue 2 , Pages 399-404, August 2004

Comparison of gene expression profiles between Peyronie's disease and Dupuytren's contracture☆

  • A Qian

      Affiliations

    • Harbor-UCLA Research and Education Institute, Torrance, California, USA
    • ,
  • R.A Meals

      Affiliations

    • Department of Orthopedics, University of California, Los Angeles, School of Medicine, Los Angeles, California, USA
    • ,
  • J Rajfer

      Affiliations

    • Harbor-UCLA Research and Education Institute, Torrance, California, USA
    • Department of Urology, University of California, Los Angeles, School of Medicine, Los Angeles, California, USA
    • ,
  • N.F Gonzalez-Cadavid

      Affiliations

    • Harbor-UCLA Research and Education Institute, Torrance, California, USA
    • Department of Urology, University of California, Los Angeles, School of Medicine, Los Angeles, California, USA
    • Corresponding Author InformationReprint requests: Nestor F. Gonzalez-Cadavid, Ph.D., Department of Urology, Harbor-UCLA Research and Education Institute, Building F-6, 1124 West Carson Street, Torrance, CA 90502, USA

Received 29 December 2003; accepted 7 April 2004.

Abstract 

Objectives

To compare the gene expression alterations in human Peyronie's disease (PD) and Dupuytren's disease (DD) to determine whether they share a common pathophysiology. Multiple mRNA expression profiles of human PD have previously shown that genes that regulate fibroblast replication, myofibroblast differentiation, collagen metabolism, tissue repair, and ossification are involved. DD, a palmar fascia fibrosis, may be associated with PD.

Methods

Total RNA samples from PD plaques, normal tunica albuginea, Dupuytren's nodules, and normal palmar fascia (nine samples per group) were subjected to differential gene expression profile analysis (Clontech Atlas DNA microarray) comparing PD with tunica albuginea and DD with normal palmar fascia. Changes of more than 2.0 in PD and DD compared with tunica albuginea and normal palmar fascia, respectively, were recorded. Reverse transcriptase-polymerase chain reactions were performed for some genes whose expression was altered in PD.

Results

Some of the gene families upregulated in both PD and DD were (a) collagen degradation: matrix metalloproteinase (MMP), with MMP2 and MMP9, and thymosins (MMP activators), with TMβ10 and TMβ4; (b) ossification: osteoblast-specific factors (OSFs) OSF-1 and OSF-2 (DD only); and (c) myofibroblast differentiation: RhoGDP dissociation inhibitor 1. The genes upregulated in PD only were decorin (an inhibitor of transforming growth factor-beta1 and a part of fibroblast replication/collagen synthesis) and early growth response protein. Reverse transcriptase-polymerase chain reaction confirmed these changes.

Conclusions

These data demonstrate that the pattern of alterations in the expression of certain gene families in PD and DD is similar, suggesting that they share a common pathophysiology and may be amenable to the same therapeutic regimens.

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☆ These studies were funded mainly by a grant from the Eli and Edythe L. Broad Foundation and in part by National Institute of Health grants R01DK-53069 and G12RR-03026.

PII: S0090-4295(04)00433-9

doi:10.1016/j.urology.2004.04.006

Urology
Volume 64, Issue 2 , Pages 399-404, August 2004

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